WebMay 29, 2024 · Phosphomolybdic Acid (PMA): Dissolve 10 g of phosphomolybdic acid in 100 mL of absolute ethanol. Potassium Permanganate: Dissolve 1.5 g of KMnO4, 10 g of K2CO3, and 1.25 mL of 10% NaOH in 200 mL of water. Vanillin: Dissolve 15 g of vanillin in 250 mL of ethanol and add 2.5 mL of conc. sulfuric acid. How does ninhydrin stain amino … WebTLC Stains Stain Uses Recipe p-Anisaldehyde General purpose stain, particularly good with groups with nucleophilic properties. Add 15 ml of AcOH and 3.5 mL of p- ... Phosphomolybdic Acid General purpose Dissolve 10 g PMA in 100 mL absolute ethanol . Title: TLC Stains.doc
Phosphomolybdic Acid: An Efficient and Easy Catalyst for …
WebTLC Stain Recipes Anisaldehyde 9.2 mL p‐methoxybenzaldehyde 3.75 mL acetic acid 338 mL 95% ethanol 12.5 mL concentrated H 2SO 4 Phosphomolybdic Acid 230 g phosphomolybdic acid 3.79 L 95% ethanol Potassium Permanganate 3 g potassium permanganate 20 g potassium carbonate 5 mL 5% sodium hydroxide WebDec 8, 2024 · With this information, you'll have the background you need for a successful TLC experiment. 4 Common Problems & Solutions for Thin Layer Chromatography Samples Problem 1: Your sample is streaking or elongated. Solution: Your sample may be overloaded. Run the separation again with a more diluted sample solution. rolling d clothing
Phosphomolybdic acid for microscopy 51429-74-4 - Sigma-Aldrich
WebPhosphomolybdic acid, ammonium salt hydrate, Thermo Scientific Chemicals. CAS: 54723-94-3 Molecular Formula: H80Mo12N3O41P-6 Molecular Weight (g/mol): 1960.994 MDL … WebApr 30, 2010 · Thin-layer chromatography was carried out on Merck aluminium sheets coated with silica gel 60 F 254. Compounds were visualized by use of 254 nm UV light and/or phosphomolybdic acid 20 wt.% solution in ethanol with heating. All solvents were purified by standard techniques . Flash chromatography was performed on Merck silica … WebApr 7, 2024 · The phosphomolybdic acid stain (PMA) is considered by some a universal stain, able to visualize a wide variety of compounds (alcohols, alkenes, alkyl iodides, and many carbonyl compounds). The yellow-green PMA reagent (Mo6 +) oxidizes the … The same underlying principles of thin layer chromatography (TLC) apply to colu… rolling cycle pcr