2024 Sftpc creert2

Sftpc creert2

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Web9 Mar 2024 · To specifically confirm which Fgfrs are expressed in mouse AEC2s, we combined the SPC + AEC2-specific Cre recombinase (Sftpc CreERT2) with the ROSA … http://www.informatics.jax.org/allele/MGI:5465199

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WebSftpc CreERT2/+;Tomato flox/+ (control), Sftpc CreERT2/+;Tomato flox/+;Fgfr2b flox/flox or Sftpc CreERT2/+; Tomato flox/+; Fgf10 +/-(experimental) mice are fed with tamoxifen food for one week followed by a chase period with normal food. Sham or left lung pneumonectomy are carried out in control and experimental mice ... Web将IRES-CreERT2敲入到小鼠Sftpc基因3'UTR中，他莫昔芬诱导型Cre重组酶CreERT2受内源Sftpc基因启动子驱动。诱导后，在II型肺泡细胞中表达Cre活性。应用领域：II型肺泡细胞Cre工具鼠 nicknames for the name ivan

Sftpc Targeted Allele Detail MGI Mouse …

WebSftpc Name surfactant associated protein C Synonyms Bricd6, pro-SpC, Sftp2, Sftp-2, SPC, SP-C Feature Type protein coding gene IDs MGI:109517 NCBI Gene: 20389 Alliance gene … Web12 Jul 2024 · SFTPC creERT2 mice were generated as previously described . To generate the Gli2 creERT2-tdT mouse, a CreERT2-2A-tdTomato expression cassette was inserted … Web19 Oct 2024 · The Sftpc-CreERT2 mouse line has been used previously to track AT2 cell fate (65, 66). Gramd2-CreERT2 mice were generated by homologous recombination insertion … now a days means

Sftpc Creert2 The Jackson Laboratory Bioz

Web10 May 2024 · Interestingly, AT2 cell proliferation at P5 occurred primarily in AT2 cells lacking the YFP lineage mark from SftpcCreERT2;R26REYFP mice ( Figures 2 D and 2E). To determine the response of AT1 cells during this injury, we lineage traced AT1 cells during hyperoxia ( Figure 2 G). Web28 May 2024 · The total AT2 population (Sftpc Pos Tom Pos cells) was isolated from lungs of 6-8 weeks old Sftpc CreERT2/+; tdTomato flox/flox mice. For the single-cell … nowadays more and more people employedWebBecause SFTPC mutations are rare (<5%) in IPF ( 14 ), a more generalized role for ER stress in AEC dysfunction and subsequent fibrosis remains to be established. The chaperone … nowadays many young people no longer choose

"WebWhen challenged with s.c. bleomycin, tamoxifen- treated Etv5 fl/-Sftpc CreERT2/+ Rosa tdTomato/+ mice tended to de- velop more extensive and severe lung lesions than control mice (Fig. 4 A-D and ... " - Sftpc creert2

Sftpc creert2

Transcription factor Etv5 is essential for the maintenance of …

WebUsing Sftpc CreERT2/+ : tdTomato flox/flox mice, we previously identified a lung populat... View Identification of a novel subset of alveolar type 2 cells enriched in PD-L1 and … http://www.informatics.jax.org/disease/DOID:1324

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Web10 Jun 2013 · Sftpc-CreERT2 lineage labels AEC2s in the adult lung. To lineage label adult AEC2s, Sftpc-CreERT2;Rosa26R-tdTm (where Tm indicates Tomato) double heterozygous mice (abbreviated Sftpc-CreER;Rosa-Tm) were given 4 doses of Tmx (0.2 mg/g body weight) and sacrificed at least 4 days after the final injection. WebCx3cr1 CreERT2 and Sall1 CreERT2 mice were crossed to two reporter strains: R26-CAG-ZsGreen and R26-YFP (Fig. 1A). In R26-CAG-ZsGreen mice, the reporter construct consists of the ZsGreen sequence placed downstream of three floxed SV40 polyA sequences (0.9 kb) that block transcription from the strong artificial CAG promotor 12.

Web29 Jul 2024 · SftpcCreERT2+/− mice were used as controls. b Genotyping of DNA extracted from CD45 (−)EpCAM (+) cells and CD45 (-)EpCAM (−) cells isolated from control and Mfn1iΔAEC2 mice 6 weeks after... Web10 Jun 2013 · For lineage-tracing AEC2s, we have used our Sftpc-CreER T2 allele in which a cassette encoding tamoxifen-activated (Tmx-activated) CreER is inserted into the …

WebOur results with the Sftpc CreERT2/+ ;Tomato flox/+ mice (PNX vs. SHAM) suggest that Fgf signaling is activated in AECII cells during PNX. Our ongoing experiments allow us to … Web3 Mar 2024 · We lineage-labeled AT2 cells using SftpcCreERT2; R26REYFP mice and subjected them to acute lung injury ( Figure 4 D). Ten days after the injury, the lineage-labeled AT2 cells expressed higher levels of AT1-specific integrins ( Itgb5 and Itgb6 ), but not the AT2-specific integrin Itga6, than uninjured control cells ( Figure 4 E).

WebGeneration of Sohlh2 CKI mice. Sohlh2 loxP/loxP and Sftpc CreERT2+ mice were both purchased from Cyagen (Suzhou, China). To generate mice with tamoxifen-inducible Sohlh2 expression speciﬁcally in AECIIs, Sohlh2 loxP/loxP were crossed to Sftpc CreERT2+ mice. To induce recombination by CreERT2, 5 consecutive intraperitoneal tamoxifen (100 …

WebMore than 99% of sorted cells expressed the AT2 cell marker Sftpc ( Fig. S1 A ). The primary AT2 cells were cultured on Matrigel for 10–12 d total, with 4-hydroxytamoxifen (4-OHT) added on the first 4 d to induce expression of Kras d12 and to delete Etv5. nowadays mesopotamia is calledWebSftpc-CreERT2小鼠与FloxP小鼠交配后，在Cre特异表达的组织或者器官或细胞中，两个FloxP位点间的外显子或元件将被删除，达到条件性敲除或过表达的目的。可用于呼吸系 … nicknames for the name heatherWeb28 Feb 2024 · The SftpccreERT2 mouse line was a gift of H. Chapman; their genotyping and generation have previously been described 34. Hopx3FlagGFP mice 35 were a gift of R. Jain and J. Epstein, and are... nicknames for the name isaacWeb3 Aug 2024 · including our recently identiﬁed AT2-SftpcLow (tdTomLow, aka injury-activated alveolar progenitors, IAAPs) [22]. In contrast to mature AT2-SftpcHigh … nicknames for the name jackieWebUsing the Sftpc CreERT2; tdTomato lineage-traced mice, we recently reported a novel AT2-IAAP subpopulation characterized by low Sftpc expression but that is enriched for PD-L1 … nowadays more and more jobs and tasksWeb3 Mar 2024 · Sftpc CreERT2 was generously provided by Chapman lab, and other strains were obtained from Jackson Laboratories (MA, USA). All experiments were performed on … nowadays more and more people keepWeb南模生物. Language: 中文 . English; 日本語; 한국어; 账户设置; 退出 nicknames for the name henrietta