Thp1 transwell
WebTHP-1 cell migration in response to CCL2. (A) THP-1 cells were seeded on the upper chamber of a transwell plate and incubated with various concentrations of CCL2 (1, 5, 10, … WebMar 30, 2024 · Last, transwell migration assays showed that IL-1β-primed SKOV3-TCM significantly increased the recruitment of THP1/PBMC Mφ, compared to control TCM and fresh medium (Figure 5I–K), and similar results were obtained using IL-1β-primed U87MG-TCM (Figure S12C–E).
Thp1 transwell
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WebThe HTS Transwell-96 permeable support has an array of 96-wells with membrane inserts connected by a rigid, robotics-friendly tray that enables all 96 inserts to be handled as a … WebTranswell permeable supports are convenient and easy-to-use devices for studies of both anchorage-dependent and anchorage-independent cells. These inserts provide …
WebMay 1, 2024 · The migration ability of THP1- cells was assayed using the transwell migration assay. Fig. 4 A shows the number of migrated cells in each group ( n = 4 in each … Webtranswell inserts are then placed into the 6 well contai ning the 2 ml medium. 3. 2 ml of the THP-1 cell suspension is added into the inserts (2 x 105 cells/insert). 4. Once settled THP …
WebJun 14, 2024 · Using a 0.4 µm Transwell coculture system (Sigma-Aldrich; Merck KGaA), THP-1 cells and TAMs were indirectly cocultured with A549 cells, respectively. A549 cells … WebSep 4, 2024 · A co-culture system of THP-1 cells and SW480 cells was established using 24-well transwell plates (Millipore). SW480 cells in the logarithmic growth phase were …
WebJul 29, 2024 · THP-1 was plated on the upper compartment of a 24-well Transwell chamber (8-μm pore diameter; Corning, NY, USA) at 1 × 10 6 cells/ml density in 200 μl fresh serum-deprived RPMI-1640 medium. Meanwhile, 800 μl CM or fresh RPMI-1640 medium alone as a blank control for a spontaneous migration were added to the lower chamber.
WebFeb 8, 2006 · A higher number of RA FLS co-cultured with differentiated THP-1 cells (1055.67 ± 63.06 cells/filter) were found to have invaded through transwell chambers than that of RA FLS co-cultured with undifferentiated THP-1 cells (726 ± 83.86 cells/filter) (P < 0.05; Figure 4b,d). indian restaurants southlake txWebApr 7, 2024 · RAGE knockdown inhibited chemotaxis and adhesion of THP-1 cells exposed to intermittent hypoxia. Knockdown of RAGE in THP-1 cells was verified via qRT-PCR (a) … indian restaurants stevenage old townWeb暨南大学,数字图书馆. 开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 indian restaurants st petersburg flWebMar 30, 2024 · The human monocyte cell line THP-1, HEK 293 T cells, human normal colon epithelial cell line NCM460 and CRC cell lines (HCT116, DLD-1, HT29, SW480, ... Wound … indian restaurants south ealingWebApr 30, 2024 · We then used the THP-1 cell line, which could be primed from monocytes to become macrophage-like cells, for the Transwell assays. Conditioned media (CM) from … indian restaurants st ives cornwallWebTranswell细胞迁移实验. 实验介绍. 细胞迁移与侵袭实验将 Transwell 小室放入培养板中,小室内称上室,培养板内称下室,上下层培养液以聚碳酸酯膜相隔,将研究的细胞种在上室内,由于 … loch gleann a\u0027 bhearraidhWeb• Working with two different cell types: Monocytes (THP1) ... DNA extraction, transfection, transwell assays, tissue sectioning using microtome, mice experiments. ... indian restaurants sunshine coast